Dynamics of homeostatic T-cell and natural killer cells activation in human blood after extracorporeal short cold incubation of cells

Frolov O.К., Litvinenko R.O., Frolova L.O., Makyeyeva L.V., Fedotov Ye.R.

1Zaporizhzhia National Universiry, Zaporizhzhia, Ukraine
2 Zaporizhzhia Medical Academy of Postgraduate Education, Zaporizhzhia, Ukraine
3 Zaporizhzhia State Medical University, Zaporizhzhia, Ukraine

We studied the dynamics of CD-structures density at intact lymphocytes of 120 persons after daily extracorporeal cold incubation. CD-phenotype was detected by spontaneous (E-RFC SP CD) and antibody - dependent monoclonal (E-RFC MAbs) rosette methods with sheep erythrocytes (SE). The density of CD-structures on lymphocytes was determined by the number of attached SE. Variations in E-RFC occurred mainly due to highly avid to SE classes of lymphocytes, which attached more than 8 SE (CL > 8 SE) , which are activated in immunogenesis. Frequency of CL>8 SE ranged from 20 to 43% in intact CD2-lymphocytes as an indicator of varying levels of stress of immune system of the surveyed individuals. The number of CL>8 SE stabilized in no statistically identical limits within 32-34 % after extracorporeal cold incubation by reducing activated (CL>8 EB) CD4+-lymphocytes with a tendency to an increase of CD8+- and CD16+-lymphocytes. Our proposed CD2-regulatory index (CD4+/CD8++CD16+) decreased from 0,98±0,11 to 0,57±0,09. We concluded that the level of CL>8 SE of CD2+-lymphocytes in the blood reflects their homeostatic activation and proliferation, and can be used as the reference value at the immune system tension detection.