Local immune response in severe atopic dermatitis and cutaneous T-cell lymphoma patients

Niyazov D.D., Fedenko1 E.S., Boldyreva M.N., Kovrigina A.M., Trofimov D.Yu.

1 Institute of Immunology, Moscow, Russia
2 N.N. Blokhin Russian Cancer Research Center, Moscow

The purpose. To investigate expression of cytokines genes parameters in skin in severe atopic dermatitis (AD) and cutaneous T-cell lymphoma (CTCL) patients comparing with healthy donors.
Materials and methods. 20 severe AD patients, 20 CTCL patients and 20 healthy donors were included in the study. Skin samples were used as material for immunological study. Interleukins -( IL)1B, IL2, IL2r, IL4, IL5, IL6, IL7, IL8, IL10, IL 12A, IL12B, IL15 (total), IL15 , IL17A, IL18, IL23, IL28, IL29, Interferon γ (IFNγ), tumor necrosis factor α (TNFα) , transforming growth factor beta 1 (TGFB1), forkhead box P3 (FOXP3) gene expression was defined in the skin of severe AD patients, CTCL patients and healthy donors by real-time reverse transcription polymerase chain reaction (RT-PCR).
Results. Studying of skin samples from CTCL patients has shown statistically significant increase of cytokines genes IL8, IL10, IL15, IFNγ expression and decrease of IL18 gene expression in comparison with skin samples from severe AD patients. Obtained expression cytokines genes parameters in skin of severe AD and CTCL patients had a certain similarities consisting of increased IL-6, IL-8, IL-10, IFNγ. Substantial differences were shown in skin of patients in comparison with healthy donors: increased IL-5, IL-12B, IL-29, TNF, TGFB1 genes expression in AD patients and increased IL-2r, IL-15 genes expression in CTCL patients. At the same time increased expression of IL-8, IL-10, IL-15, IFNγ genes in CTCL patients in comparison with AD patients was established.