Glomerular filtration rate, readiness to apoptosis and late apoptosis of peripheral blood lymphocytes in different variants of bronchial asthma

Mineev V.N., Vasilyeva T.S., Nesterovich I.I.

First Saint­Petersburg State Medical University named after academician I.P. Pavlov, St. Petersburg, Russia

The aim of the study is to establish the possible influence of apoptosis of peripheral blood lymphocytes (readiness to apoptosis and late stage of apoptosis) on glomerular filtration rate in different variants of bronchial asthma (BA).
Materials and methods. 215 patients with different variants of BA were examined. Glomerular filtration rate (eGFR) by CKD-EPI was estimated. Activity the spontaneous expression of the receptor CD95/Fas/APO-1 on lymphocytes were evaluated using monoclonal antibodies of the company Immunotech (Coulter Corp., USA). To determine the late stage of lymphocytes apoptosis we used a set of firm Becton Dickinson (Germany) comprising monoclonal antibodies to active caspase-3 conjugated to phycoerythrin.
Results. Glomerular filtration rate is significantly reduced in non-allergic BA (NABA) group and in the group of patients with asthma receiving systemic (oral) therapy with glucocorticosteroids (GCBA) in comparison with the group of patients with allergic BA (ABA).
The expression of CD95/Fas/APO-1 receptor and caspase 3 in peripheral blood lymphocytes was significantly increased in NABA and GCBA as compared with the group of ABA patients.
Factor analysis revealed that in ABA glomerular filtration rate was not related with spontaneous lymphocytes apoptosis (both at the stage of readiness of cells to him, and at the late
stage of apoptosis). In contrast, in NABA and GCBA the degree of the factor loadings of apoptosis in factor model, predominantly the degree of late stage (caspase 3) was very significant in relation to glomerular filtration rate.
Conclusion. Lymphocytes apoptosis (cells readiness to apoptosis - CD95/Fas/APO-1 receptor and especially its late - stage caspase 3) is likely participate in the decrease of glomerular filtration rate in NABA and GCBA and it is considered as an additional risk factor for CKD in this variant of the disease.