The impact of fetal load of biologically active substances of Hirudo verbana saline extract antigens on the morphometric and hematologic parameters of rats in the early stages of post-embryonic development

Aminov R.F., Frolov A.K., Fedotov Y.R.

Zaporizhzhia National University, Zaporizhzhia, Ukraine

The female rats of wild type 2 weeks before and 2 weeks after mating were administered intraperitoneally medicinal leech saline extract antigens (4 injection). Studied females after feeding the offspring and the offspring itself in dynamics at 1, 15, 30, 60 days. All animals were decapitated under anesthesia after measuring morphometric parameters of the body. Then we did the autopsy animals and studied morphometric parameters of immunogenic organs (spleen and thymus), laboratory parameters: total white blood cells count, red blood cells count, hemoglobin, color index, leukocyte formula. As a result of our investigations was found an immunostimulatory effect of antigens from saline extract of the medicinal leech on morphometric parameters of the body and major immunogenic organs of rats. Under the influence of medical leeches’ antigens from the first day increased the number of white blood cells, increased the number of red blood cells, increased hemoglobin, color index was in the normal range. Changes of morphological and hematological parameters indicate the immune-stimulating impact of medical leeches’ antigens on leucopoiesis, erythropoiesis and morphogenesis.