Study of the specific activity of the granulated form of the mixed-allergen from house dust mites Dermatophagoides pteronyssinus and Dermatophagoides farinae for oral immunotherapy

Berzhets V.M., Koreneva S.V., Khlgatian S.V., Emelyanova O.Yu., Petrovа S.Yu., Vasilyeva A.V.

Mechnikov Research Institute of Vaccines and Sera, Moscow

Objective: To study the properties of the granulated form of the mixed-allergen from Dermatophagoides pteronyssinus (Der.p.) and Dermatophagoides farinae (Der.f.). Methods: Immunodot was used for determination of allergens in the experimental preparation. Guinea pigs were immunized to study the immunogenic properties of the experimental allergen (ЕА), this was followed by the study of animal blood serum by ELISA. Results: It has been proved, that the granulated form of the mixed-allergen from Der.p. and Der.f. has an allergen specific activity. The ЕА causes a statistically significant increase of specific IgG antibodies to Der.p. and Der.f. among immunized Guinea pigs compared to the control group. Conclusion: The results indicate possible therapeutic efficacy of the ЕА in sublingual immunotherapy.