Modeling of bacterial lipopolysaccharide-induced endometritis in rats and the possibility of its therapy using plasma enriched with soluble platelet factors

Potapnev M.P., Pavlov K.I., Doronina O.K., Metelitsa T.G., Anfinogenova E.A., Davydov D.A., Chegodaeva E.V., Kurklinskaya G.A., Naborovskaya A.M.

Belarusian State Medical University, Minsk, Belarus
Peoples Friendship University of Russia (RUDN University), Moscow, Russian Federation

We presents results of a study on endometritis modeling in rats with double intravaginal administration of bacterial lipopolysaccharide (LPS). The established endometrial inflammatory process was accompanied by a decrease in the weight of animals, an increase in the weight of the uterus, neutrophilic infiltration, edema, and overexpression of cyclooxygenase in endometrium on 30-45 days after the start of endometritis induction. Local intravaginal use of plasma enriched with soluble platelet factors/platelet-rich plasma (PORFT/PRP) caused a decrease in the intensity of the inflammatory process and the rate of neutrophil infiltration of the endometrium, a decrease in the intensity of cyclooxygenase, interleukin-6 and transforming growth factor-β expression, and an increase in the body weight of animals. The conclusion was made on the possibility of creating model of endometritis in rats by double administration of LPS and the ability of local administration of PORFT/PRP to have a therapeutic effect on the inflammatory process in the endometrium.