Detection of neutrophil extracellular traps formation using the hexaazotated fuchsin and determination of the immunoglobulin classes A and M abzyme activity in patients with bronchial asthma and COPD

Generalov S.I., Ishchenko O.V., Sushkova S.A., Generalov I.I.

Vitebsk State Medical University, Vitebsk, Belarus

Netosis and abzyme activity are the significant components of the innate immunity. Spontaneous and induced netosis, various types of proteolytic and oxidoreductase abzyme activity in patients with COPD and BA were determined in this study. NETs were determined by both light and fluorescence microscopy using a previously developed staining method. As a result of the work, an increase of the induced netosis was found in patients with COPD, relative to patients with BA. A significant increase in the IgA peroxidase activity was found in patients with BA relative to patients with COPD. Data on hyaluronidase, catalase, elastase, dipeptidyl dipeptidase-4-similar activity were obtained. A correlation between the peroxidase and catalase activity of IgA in patients with BA was found.