Analysis of the resistance of clinical isolates of Escherichia coli to antimicrobial agents in single-centre study

Tsуrkunova Zh.F., Emelyanova А.А., Gudkova E.I., Skorokhod G.A., Gavrilova I.A., Butkevich V.V., Dolgina Yu.N., Vashkevich M.P., Shepelevich N.A.

Belarusian state medical university, Minsk
N.N. Alexandrov national cancer centre of Belarus, Minsk

The aim of this research is to assess the phenotypic resistance to antimicrobial agents of clinical isolates of Escherichia coli from patients undergoing treatment in various departments of the oncological clinic in Minsk. In this work, 34 clinical isolates of Escherichia coli were studied. Species identification and determination of sensitivity to antibiotics was performed on a Vitek2Compact analyzer (BioMerieux). The resistance / sensitivity of clinical bacterial isolates to antiseptics was determined by the agar dilution method. The profiles of phenotypic resistance / sensitivity of 34 clinical E. coli isolates to 15 antibiotics and 8 antiseptics were determined. Based on the results of a comparative analysis, 5 bacterial isolates were assigned to one sensitivity profile. 80-100% of the studied bacterial isolates were susceptible to amoxicillin/clavulanic acid, ertapenem, imipenem, meropenem, amikacin, gentamicin, fosfomycin, nitrofurantoin. 26-42% of the E. coli isolates showed resistance to cefotaxime, ceftazidime, cefepime, ciprofloxacin and norfloxacin. Multiple resistance (to 4 antibiotics or more) was characteristic for 35.3% of the studied isolates of microorganisms. One strain of E. coli isolate was resistant to 11 studied antibiotics. Analysis of the resistance of clinical E. coli isolates to antiseptics showed that iodinol, iodiskin, septomyrin were ineffective in inhibiting bacterial growth on an agar plate. A high level of antimicrobial activity against E. coli was found in the antiseptic agents chlorhexidine bigluconate and hydrogen peroxide. High efficacy of amoxicillin/clavulanic acid, ertapenem, imipenem, meropenem, amikacin, gentamicin, fosfomycin, nitrofurantoin and low efficacy of cefotaxime, ceftazidime, cefepime, ciprofloxacin, norfloxacin against clinical isolates of E. coli circulating in the oncological clinic of Minsk was revealed. A phenotypic resistance of E. coli to pharmaceutical forms of antiseptics iodinol, iodiskin and septomyrin was established.