Characteristics of structural changes in the intestine, microbiocenosis and amino acid pool after administration of ertapenem

Lemesh A.V., Nikolaeva I.V., Doroshenko E.M., Ostrovskaya O.B., Sheybak V.M., Yakubtsevich R.E.

Grodno State Medical University, Grodno, Belarus
Grodno University Clinic, Grodno, Belarus

Аim of the study. To analyze the effect of Ertapenem on the pool of free amino acids, microbiota and intestinal structure.
Materials and methods. The study was carried out on 5 animals from the control group and 6 animals from the experimental group. Animals in the control group were intraperitoneally injected with a 0.9% NaCl solution for 10 days; Animals in the experimental group were intraperitoneally administered Invanz (ertapenem) at a dose of 60 mg/kg for 10 days. Immediately after decapitation, the abdominal cavity of the rat was opened, a section of the small intestine was collected, and the samples were fixed in Carnoy's fluid. Determination of free amino acids in plasma and microbial-tissue complex of blood was carried out by reverse-phase HPLC with o-phthalaldehyde and 3-mercaptopropionic acid with isocratic elution and fluorescence detection.
Results and discussions. Morphometric analysis of the intestines in the ertapenem group showed a slight decrease in mucosal thickness and crypt depth. In half of the animals, in the apical regions of some villi, a pronounced expansion of blood capillaries with stasis of erythrocytes was observed, in places accompanied by the marginal standing of lymphoid cells. The microbiocenosis was characterized by an increase in the number of aerobes and spore-forming anaerobes, and the absence of lactose-positive bacteria of the Escherichia coli group. The study of the amino acid fund and the spectrum of individual amino acids in the microbial-tissue complex of the small intestine showed a significantly significant increase in aspartate, glycine, as well as a decrease in hypotaurine.
Conclusion. The effect of ertapenem led to local mild inflammatory changes in the villous stroma, a moderate decrease in the formation and extrusion of mucus by goblet cells of the villi and crypts. Amino acid changes that occur after the action of ertapenem may be due to both the intestines and changes in the microbiota.