Structure of gut microbiocenosis in newborn children associated with ESBL-producing Klebsiella pneumoniae at the stage of nursing in a hospital condition

Ustyuzhanin A.V., Chistyakova G.N., Remizova I.I., Makhanyok A.A.

Ural Scientific Research Institute of Maternity and Child Care, Yekaterinburg, Russia

Introduction. An active search is underway of strains potentially useful for probiotic preparations with vivid antagonistic properties that prevent the adhesion and proliferation of multidrug-resistant bacteria.
The aim of this study was to study the structure of the intestinal microbiocenosis of newborn children associated with ESBL-producing Klebsiella pneumoniae at the stage of nursing in a hospital.
Materials and methods. We studied fecal samples obtained from 21 newborns whose intestines were colonized by ESBL-producing strains of K. pneumoniae. The age of children ranged from 3 to 58 days (21.18+5).
Results. All samples contained DNA from bacteria of the order Enterobacterales, which is explained by the selection of fecal samples from children with K. pneumoniae identified in preliminary testing. DNA of Bifidobacterium spp. was detected in 19 out of 21 fecal samples (90.4%), while DNA of Bifidobacterium bifidum was detected in 14 cases (66.6%), Bifidobacterium animalis subsp – in 11 (52.4%). DNA of Enterococcus spp. detected in 18 samples (85.7), Staphylococcus spp. – in 13 (61.9%), Streptococcus spp. – in 11 (52%), Dialister spp./Alisonella spp./Megaspherae spp./Veillonella spp. – in 9 (42.9%), E. coli – in 2 (9.5%). DNA of Candida spp. was isolated once non-albicans, Methanobrevibacter spp. Lactobacillaceae, Erysipelotrichaceae, Clostridium perfringens gr.
Conclusion. DNA detection of Bifidobacterium spp. and ESBL-producing K. pneumoniae indicates insufficient protection of the intestinal biotope by bifidobacteria from bacteria of the ESCAPE group, which may be due to the initial colonization of K. pneumoniae, followed by the addition of bifidobacteria. At the same time, the problem of preventing the spread of infectious agents associated with the provision of medical care and their colonization of non-sterile loci in the body of a newborn child at the inpatient stage of medical care remains unresolved and requires further study and development of effective measures.