Nuclease polyclonal IgG activity in patients with early rheumatoid arthritis

Volkova M.V., Kunder E.V., Generalov I.I.

The nuclease activity of polyclonal IgG to various substrates (a bacterial plasmid double-stranded DNA E.coli, DNA of Chlamydia trachomatis, obtained by the amplification calf thymus DNA (Sigma) in patients with early rheumatoid arthritis. The highest IgG activity to calf thymus DNA (Sigma) was revealed. We assessed the DNAse IgG activity against calf thymus DNA (Sigma) in 63patients with early rheumatoid arthritis and 45 healthy donors.
The levels of DNAse IgG activity in patients with early rheumatoid arthritis were higher (p<0,001) in comparison with the same ones in healthy persons. We revealed the multiply relationships between DNAse polyclonal IgG activity levels, clinical symptoms of early rheumatoid arthritis and laboratory findings in examined patients.
The detection of high DNAse IgG activity levels in patients with early rheumatoid arthritis confirms the role of abzymes in the immune pathogenesis of this disease.